AI/ML-based REAL Libraries: Unlocking New Frontiers in Protein Degradation
1 600 compounds CRL E3 Library
1 520 compounds HECT E3 Library
1 520 compounds RBR E3 Library
The success of high-throughput screening in identifying lead compounds for drug discovery depends on the quality and specificity of the compound library. Our Focused E3 Ligase Library, designed in collaboration with Recursion and powered by MatchMaker, provides researchers with high-quality compounds tailored for targeting key E3 ubiquitin ligase families: Cullin-RING Ligases (CRLs), HECT Ligases, and RBR Ligases. These libraries contain a carefully curated selection of compounds that facilitate the modulation of these crucial enzymes, opening new avenues for targeted protein degradation and drug discovery.
These libraries were designed to aid in the discovery of novel therapeutics for diseases involving dysregulated protein homeostasis. Targeting CRLs and HECT ligases to modulate pathways involved in tumor suppression and oncogenesis. RBR ligases, such as Parkin, play a key role in diseases like Parkinson’s and Alzheimer’s by regulating mitochondrial quality control and protein aggregation. Modulating immune response pathways through selective targeting of E3 ligases involved in inflammation and immune signaling. Exploiting the ubiquitin-proteasome system to identify new antiviral and antibacterial therapeutic strategies.
Enamine continuously expands its screening catalog with innovative compounds built upon cutting-edge chemistry, ensuring that our libraries remain at the forefront of modern medicinal chemistry.
Typical Formats
Catalog No.
CRL-E3-1600-Z-2
Compounds
1 600
2 plates
Amount
≤ 300 nL of 2 mM of DMSO solutions
Plates and formats
1536-well Echo Qualified microplates (001-6969 LP-0400)
Price
Catalog No.
HECT-E3-1520-Y-10
Compounds
1 520
5 plates
Amount
10 µL of 10 mM DMSO solutions
Plates and formats
384-well Echo Qualified LDV microplates (001-12782 LP-0200)
Price
Catalog No.
RBR-E3-1520-Y-10
Compounds
1 520
5 plates
Amount
50 μL of 10 mM DMSO solutions
Plates and formats
384-well Greiner Bio-One microplates (781280)
Price
*We will be happy to provide our library in any other most convenient for your project format. Please select among the following our standard microplates: Greiner Bio-One 781270, 784201, 781280, 651201 or Echo Qualified 001-12782 (LP-0200), 001-14555 (PP-0200), 001-6969 (LP-0400) or send your preferred labware. Compounds pooling can be provided upon request.
Download SD files
1 600 compounds
1 520 compounds
1 520 compounds
Key features
- AI/ML-Driven Selection – Molecules in each library selected with AI/ML tool MatchMaker based on predicted binding/matching pairs to protein targets.
- Novel Compounds – Recently synthesized and designed for E3 ligase modulation.
- MedChem-Friendly – Carefully filtered to exclude PAINS and undesirable functionalities.
- Follow-up Readily Available – All compounds originate from Enamine’s REAL Space, ensuring seamless access to rapid resynthesis and analog searches for structure-activity relationship (SAR) studies, expediting lead optimization and hit expansion.
- High Purity Compounds – All compounds have passed rigorous HPLC analysis and are at least 90% pure.
Examples of the molecules in the library
CRL E3 Library
HECT E3 Library
RBR E3 Library
Unlock new possibilities in targeted protein degradation and E3 ligase modulation with our Focused E3 Ligase Libraries.