Curated selection of frequent hitters
83 compounds
Nuisance compounds are commonly known for their interference with bioassay readouts in high-throughput screening (HTS), causing false positives that are inapplicable to the hit-finding process. Recently, Jonathan Baell provided a description of nuisance compound classes, including colloidal aggregates, PAINS (Pan-Assay Interference Compounds), electrophiles and redox cyclers, chelators, as well as optical and phenotypic assay interference compounds.
We provide a curated list of more than 80 nuisance chemicals known to obstruct assay readouts in target-based and phenotypic screenings. Conveniently available in an assay-ready screening plate, this nuisance compound set is expected to be of great interest to the research community, helping to develop high-quality HTS assays that yield promising, optimizable hits. Hits arising from this small set of nuisance compounds would be highly informative in screening campaigns, assisting target-owners in identifying the types of interference compounds that their assays might be susceptible to.
Most popular library formats available for immediate supply
Catalog No.
CONS-0-Z-10
Compounds
83
1 plate
Amount
≤ 300 nL of 10 mM of DMSO solutions
Plates and formats
1536 well, Echo Qualified 001-6969 (LP-0400), first four and last four columns empty, 1280 compounds per plate
Price
Catalog No.
CONS-10-Y-10
Compounds
83
1 plate
Amount
10 µL of 10 mM DMSO solutions
Plates and formats
384 well, Echo Qualified LDV microplates 001-12782 (LP-0200), first and last two columns empty, 320 compounds per plate
Price
Catalog No.
CONS-50-X-10
Compounds
83
2 plates
Amount
50 μL of 10 mM DMSO solutions
Plates and formats
96 well, Greiner Bio-One microplates 781280, first and last columns empty, 80 compounds per plate
Price
*We will be happy to provide our library in any other most convenient for your project format. Please select among the following our standard microplates: Greiner Bio-One 781270, 784201, 781280, 651201 or Echo Qualified 001-12782 (LP-0200), 001-14555 (PP-0200), 001-6969 (LP-0400) or send your preferred labware. Compounds pooling can be provided upon request.
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Key features
- Enhances HTS assay reliability by addressing potential interference
- Over 40 different modes of action
- Readily accessible in an assay-ready screening plate
- References for compounds are available
Library design
The global literature overview and data analysis served as the foundation for the design. Over 80 compounds are categorized based on their specific targets, structural elements, and types of interactions. The collection includes compounds widely recognized and validated as promiscuous across a range of independent and distinct screening methodologies.
Example of structures
