Special diversity library created for Phenotypic Screens
5 760 compounds
Phenotypic screening has proven its efficacy in drug discovery and become more and more popular approach in search of new actives. Recently researchers have concluded that sharp focused approaches such as target-based screening are useful but may also limit the breadth of new findings.
Development of new phenotypic screening techniques within implementation of specially designed compound libraries makes this approach a powerful tool in repurposing, finding of new mechanism of action, investigation of signaling pathways and discovery of new biological targets. For successful screening campaign special attention must be paid to the source of chemical entities and their annotation. This requires access to richer, diverse and cross-linked biological data associated to chemical compounds. In order to meet all requirements, we designed special compound library intended for phenotypic screens.
The Library has been pre-plated for most convenient access and prompt delivery in various customized formats. Most popular library formats available for immediate supply are summarized in the table below:
Typical Formats
Phenotypic Screening Library is available for supply in various pre-plated formats, including the following most popular ones:
Catalog No.
PSL-5760-0-Z-10
Compounds
5 760
5 plates
Amount
≤ 300 nL of 10 mM of DMSO solutions
Plates and formats
1536-well Echo LDV microplates, first and last four columns empty, 1280 compounds per plate
Price
Catalog No.
PSL-5760-10-Y-10
Compounds
5 760
18 plates
Amount
≤ 10 µL of 10 mM DMSO solutions
Plates and formats
384-well, Echo Qualified LDV microplates #001-12782 (LP-0200), first and last two columns empty, 320 compounds per plate
Price
Catalog No.
PSL-5760-50-Y-10
Compounds
5 760
18 plates
Amount
50 μL of 10 mM DMSO solutions
Plates and formats
384-well, Greiner Bio-One plates #781280, 1,2 and 23,24 columns empty, 320 compounds per plate
Price
Catalog No.
Library & follow-up package
Plates and formats
PSL-5760-10-Y-10 screening library 5 760 cmpds, hit resupply, analogs from 4.4M+ stock and synthesis from REAL Space
Price
*We will be happy to provide our library in any other most convenient for your project format. Please select among the following our standard microplates: Greiner Bio-One 781270, 784201, 781280, 651201 or Echo Qualified 001-12782 (LP-0200), 001-14555 (PP-0200), 001-6969 (LP-0400), C52621 or send your preferred labware. Compounds pooling can be provided upon request.
Download SD files
Library Design
To create multipurpose phenotypic library we investigated an optimal balance between diversity of biological activities versus structural diversity of small molecules. The library includes 900+ approved drugs and most similar compounds with identified mechanism of action (T>85%, linear fingerprints). In addition, PSL is enriched with 2000+ of annotated potent inhibitors and their biosimilars, covering a broad diversity of biological targets. Compounds from PSL are cell-permeable and possess pharmacology-compliant physicochemical properties.
- Approved drugs and most similar compounds with identified mechanism of action, over 2 000 molecules, that were found from Enamine Collection (T>87%, linear fingerprints).
- Potent inhibitors or highly similar compounds to diversified protein targets, about 5 000 molecules.
Supporting biological data/documentation:
- Provides easy access and analyze information from in-fields.
- Covers different aspects: polypharmacology, number of targets and description.
- Numbers and names of associated diseases are indicated for majority of compounds.
Figure below demonstrates chemical space distribution using 2D prop parameters (ClogP, fraction of Rotatable bonds, donor/acceptor count, etc.), normalized PMI vectors and distribution between classes of the most potent targets, total number of targets per compound and first level of Anatomical Therapeutic Chemical (ATC) Classification.
Our Phenotypic Library can be applied for screening against different protein classes and diseases, affecting adjacent tissues or individual body systems.
