Special diverse selection of frequent hitters
320 compounds
Pan-Assay Interference Compounds (PAINS) are the most recognized filters among medicinal chemists. Since first publication in 2010 by John Bell these filters have become industry standard in Drug Discovery. While carefully removing all PAINS-related compounds from Enamine libraries we realized that these compounds can be very useful in HTS assay set-up & validation.
Special diversity set of PAINS available for prompt delivery in various plated formats including the most popular listed below:
Typical Formats
PAINS Library is available for supply in various pre-plated formats, including the following most popular ones:
Catalog No.
PAINS-320-0-Z-10
Compounds
320
1 plate
Amount
≤ 300 nL of 10 mM of DMSO solutions
Plates and formats
1536-well Echo LDV microplates, first and last four columns empty, 1280 compounds per plate
Price
Catalog No.
PAINS-320-10-Y-10
Compounds
320
1 plate
Amount
≤ 10 µL of 10 mM DMSO solutions
Plates and formats
384-well, Echo Qualified LDV microplates #001-12782 (LP-0200), first and last two columns empty, 320 compounds per plate
Price
Catalog No.
PAINS-320-50-Y-10
Compounds
320
1 plate
Amount
50 μL of 10 mM DMSO solutions
Plates and formats
384-well, Greiner Bio-One plates #781280, 1,2 and 23,24 columns empty, 320 compounds per plate
Price
Catalog No.
Library & follow-up package
Plates and formats
PAINS-320-10-Y-10 screening library 320 cmpds, hit resupply, analogs from 4.4M+ stock and synthesis from REAL Space
Price
*We will be happy to provide our library in any other most convenient for your project format. Please select among the following our standard microplates: Greiner Bio-One 781270, 784201, 781280, 651201 or Echo Qualified 001-12782 (LP-0200), 001-14555 (PP-0200), 001-6969 (LP-0400), C52621 or send your preferred labware. Compounds pooling can be provided upon request.
Download SD files
Library design
The design is based on substructural motifs of known PAINS compounds. We used originally reported PAINS filters to identify a set of over 80k in-stock compounds. This set was clustered using fingerprint-based approach and Tanimoto similarity distance calculations. The most populated clusters with at least 5 compounds were extracted and one representative example was selected from each cluster resulting in a library of 320 compounds. The library of most diverse 320 PAINS is available in plate format for your convenience. Examples are given below.
Key features
- Represents the most common false positives
- Substructure & scaffold diversity
- All compounds suitable for storage as DMSO solutions
Example of structures and their specific PAINS filters
