Structurally optimized tetrazines for rapid biological labeling

Bioorthogonal chemical reactions are closely associated with the characteristics of “click” chemistry, occurring with high selectivity and fast reaction kinetics in vivo. Consequently, these reactions found use as multipurpose tools for chemical biology. The Inverse-electron-Demand Diels–Alder (iEDDA) reaction between tetrazines and strained alkenes is fairly new ligation reaction, which displays rates 3-7 orders of magnitude faster than many bioorthogonal reactions. High reaction rates, biocompatibility, together with the ability of tetrazines to quench fluorescence of some fluorophores, widely used for fluorescent labeling, and recover it after iEDDA reaction (Figure 1) make tetrazine derivatives unique and versatile tools for bioortogonal chemistry. Figure 2 is showcasing possible approach to modification of commonly used fluorophore as fluoresceine (A) with tetrazines and application of tetrazine derivatives in DNA encoded libraries technologies (DELT), as the core scaffolds (B).

Figure 1. General scheme of IeDDA ligation.

Figure 2. Tetrazine ligation of fluoresceine (A) and use of tetrazine core in DELT-compatible reaction (B).

We offer:

Herein, we propose a diverse library of tetrazine derivatives, carefully selected to match the widest scope of chemical biology challenges (> 30 compounds in stock). Moreover custom synthesis of tetrazine building blocks is available.

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